浩瀚色譜采用毛細(xì)管柱氣相色譜法測定植物油中脂肪酸組成。實(shí)驗(yàn)中選用氫氧化鉀-甲醇溶液作為甲酯化試劑對樣品進(jìn)行處理,再用正己烷提取,通過氣相色譜儀對脂肪酸組成進(jìn)行分離和鑒定。總共分離出15種脂肪酸,結(jié)果表明:植物油中的脂肪酸以棕櫚酸、硬脂酸、油酸、亞油酸為主;只有胡麻油中油酸和亞油酸相對含量為31.7%,其他四種植物油油酸和亞油酸含量均高于75%;而菜籽油中的油酸相對含量最高,大豆油中的亞油酸相對含量最高;五種植物油中不飽和脂肪酸含量高于飽和脂肪酸含量,其中菜籽油中不飽和脂肪酸相對含量最高。
名稱:毛細(xì)管柱
型號:HH-PEG-20M
規(guī)格:30m*0.32mm*0.25um
*高溫度:250°C
編號:20240514009
應(yīng)用:植物油在脂肪酸的測定 氣相色譜法
適用于安捷倫6890,7890,7820,8860,8890；島津GC-2014，GC-2018，GC-2030，GC-2010
Haohan chromatography uses capillary column gas chromatography to determine the fatty acid composition in vegetable oil. In the experiment, potassium hydroxide methanol solution was selected as the methylation reagent to treat the sample, followed by n-hexane extraction. The fatty acid composition was separated and identified by gas chromatography. A total of 15 fatty acids were isolated, and the results showed that the main fatty acids in vegetable oil were palmitic acid, stearic acid, oleic acid, and linoleic acid; Only the relative content of oleic acid and linoleic acid in sesame oil is 31.7%, while the content of oleic acid and linoleic acid in the other four plant oils is higher than 75%; The relative content of oleic acid in rapeseed oil is the highest, while the relative content of linoleic acid in soybean oil is the highest; The content of unsaturated fatty acids in five types of vegetable oils is higher than that of saturated fatty acids, with rapeseed oil having the highest relative content of unsaturated fatty acids.
Name: Capillary Column
Model: HH-PEG-20M
Specification: 30m * 0.32mm * 0.25um
*High temperature: 250 ° C
Number: 20240514009
Application: Vegetable oils - Determination of fatty acids - Gas chromatography method
Applicable to Agilent 68907890782088608890; Shimadzu GC-2014, GC-2018, GC-2030, GC-2010